Phase I Calibration Findings

During NIH-funded Phase I research, Liquen Technologies demonstrated the feasibility of a compact, portable calibration system using supercritical CO₂ cartridges and dynamic dilution. Our findings support accurate delivery of trace gases and semi-volatile compounds, advancing reliable on-site calibration without bulky compressed-gas cylinders.

A laboratory workbench with scientific equipment including glassware, test tubes, papers, and electronic devices, in a well-lit lab with wooden shelves and white cabinets.

Gas Calibration

This image presents three graphs illustrating calibration data for different gases: carbon monoxide (CO), nitrogen dioxide (NO₂), and sulfur dioxide (SO₂). Each graph shows a linear relationship between measured and true concentrations with high accuracy, indicated by R² values close to 1. The top graph displays calibration for CO, the bottom left for NO₂, and the bottom right for SO₂. There is a note at the bottom mentioning R² values ranging from 0.9986 to 0.9999 across all gases, and the brand name 'LIQUEN'.

  • Calibration results for carbon monoxide, nitrogen dioxide and sulfur dioxide were highly linear, with coefficients of determination of 0.999 or greater.

  • Cartridge-based BTEX standards closely matched a conventional compressed-gas reference. Calibration-slope ratios were 1.01 for benzene, 1.02 for toluene and 1.04 for ethylbenzene and xylenes.

  • The carrier fluids produced no significant sensor response or change in sensitivity at the tested dilution levels.

A woman in a gray sweater uses a medical device connected to a glass container with green liquid, set up on a tabletop in a bright room with large windows.

Particles

Our early particle testing produced a steady flow of calibration particles and identified the improvements needed to make future measurements more precise and repeatable.

Industrial pipes and valves with water flowing into a metal container in a factory or plant setting.

PFAS

Verifying PFAS destruction where treatment occurs.

PFAS treatment teams still rely heavily on costly, laboratory-based standards and time-consuming sample analysis. Emerging portable instruments can also drift and often require frequent calibration. Liquen’s goal is to provide compact, traceable gas-phase standards that help verify analytical instruments before and during PFAS destruction trials